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HCEMM-SU In Vivo Imaging ACF
Home（https://www.hcemm.eu/） > Services（https://www.hcemm.eu/services/） > Knowledge Transfer（https://www.hcemm.eu/services/knowledge-transfer/） > HCEMM-SU In Vivo Imaging ACF
In Vivo Imaging（https://www.hcemm.eu/services/knowledge-transfer/HCEMM-SU-In-Vivo-Imaging-ACF/）
Singe-cell Omics（https://www.hcemm.eu/services/knowledge-transfer/hcemm-brc-singe-cell-omics-acf/）
Functional Cell Biology and Immunology（https://www.hcemm.eu/services/knowledge-transfer/HCEMM-USZ-Functional-Cell-Biology-and-Immunology-ACF/）
HCEMM-SU In Vivo Imaging Advanced Core Facility
Location and Equipment
The IVI ACF is located in three sites, the headquarters being the Basic Medical Sciences Building at the Semmelweis University, 37-43 Tűzoltó utca, 1094 Budapest . The ACF HQ comprises two isotope laboratories and three imaging laboratory rooms. Permissions are in place to handle more than 80 isotopes for tracing, imaging and therapeutics and X-ray appliance. In addition, a local isotope-containing animal facility is also associated with the ACF. Two other sites are also involved, the Cardiovascular Centre (68 Városmajor u, 1122 Budapest) and the Basic Sciences Centre (4 Nagyvárad tér, 1082, Budapest).
Available Equipment for Molecular Imaging
Small Animal Ultrahigh Resolution Optoacoustics and Doppler Ultrasound Imaging System: Iconeus ONE (Iconeus, France)
Small Animal Ultrahigh Resolution Ultrasound System: Vevo 3100 (Fujifilm, Canada)
Translational High Resolution Ultrasound System: Vivid E95 (GE, US)
Dedicated Clinical SPECT/PET/CT System for large animals: AnyScan TRIO (Mediso, Hungary)
Optical Imaging Systems: FOBI (Neoscience, Korea)
Available Equipment for Structural Micro-Imaging
Ex vivo Micro-CT System: Skyscan 1272 (Bruker, Belgium)
Additional Equipment
PET/MRI system: nanoScan PM 3T (Mediso, Hungary).
SPECT/CT system: nanoScan SPECT/CT 4R (Mediso, Hungary).
Services
The ACF provides services in a broad range of in vivo imaging applications using an already existing infrastructure, which is continuously being expanded. For the usually needed small animal imaging studies presumably in the forefront of possible users, the ACF has an immediate access to multiple animal models (optimized at conventional and SPF animal facilities) such as e.g. inflammation models (including collagen-induced arthritis, autoantibody-induced arthritis, various dermatitis models), cardiac and metabolic disease models (including acute and chronic ischemia-reperfusion, heart failure, hypertonia, atherosclerosis, diabetes, hyperlipidemia, obesity, uremia, cardiogenic shock) as well as various transgenic and humanized mice. Tumour xenografts including a variety of cell lines are also available based on user-borne cost and maintenance models.
The ACF offers a comprehensive service to its users. In this section we outline options users are able to utilize in their projects.
Choice of imaging measurements and dimensions for the users’ given experimental questions.
Choice of one or multiple imaging methods, selection of the applicable animal models and proposed imaging contrast materials.
Experimental and statistical planning of image acquisition measurements.
Planning of data analysis and if needed, radiomics generation methods.
Short-time (up to 30 days) hosting of rats or mice in a separate animal house in the Facility.
Generation of the animal model(s).
Application of contrast materials or experimental treatments in all routes.
Animal anaesthesia and image acquisitions.
Storage and export/import of image data into common biomedical file formats: DICOM 3.0, RAW, Analyze 7.5, NIfTI, and different image file formats such as png, jpg, img, or bmp.
Analysis of data files by creating quantitative Volume of Interest (VOI) data and exporting them to .csv or other spreadsheet readable formats.
Biomedical scientific interpretation of visualised images and time series, data sorting and interpretation
When specifically agreed, statistical methodology and evaluation of the generated data.
The Facility obtains an imaging ethical permission for use of rats and mice. Mild-level animal imaging experiments (only anaesthesia and dosing routes of contrast agents considered mild) are readily available.
Essentially, the complexity of the above technological platform enables the ACF to offer an unprecedented and highly flexible solution to statically, dynamically, and functionally image all organ systems of the given organism. Moreover, it also provides an excellent technological arsenal for applications in multiple scientific fields of e.g. pharmacology, molecular oncology, histology, inflammation research, biomarker identification and detection – in parallel with assessing physiological and pathophysiological phenomena.